Assessment of Serum Gonadotrophins and Prolactin Hormone Levels in In Vitro Fertility (IVF) patients in selected fertility clinics in Port harcourt, Nigeria: A measure of Relevance in Assisted Reproduction Technology (ART)
The study was designed to determine the levels of gonadotrophins ( luteinizing hormone (LH), follicle stimulating hormone (FSH)) and prolactin in women attending fertility clinics and were about to begin In vitro fertility treatment. Sixty (60) women who attended In vitro fertility clinic in various private fertility hospitals in Port Harcourt were used for the study. These hormonal tests were part of preliminary investigations carried out on the patients before the treatment. As a routine, the
... . As a routine, the consent of the women was obtained for the investigations to be carried out on their samples. Fasting blood samples were collected on day two (2) of their menstrual cycle, and serum hormone (FSH, LH and Prolactin) concentrations analyzed by ELISA (Enzyme-linked immunosorbent assay) method with automated micro plate reader. The subjects were married for at least two years; and were within the child bearing age of 35-44years. Out of 60 patients examined for LH, FSH and prolactin concentrations, 29 (48.3%) had normal hormonal concentrations, 31(51.7%) had elevated hormone levels represented as follows:3(5%) had elevated levels of both LH,FSH and prolactin,1(1.7%) had elevated levels of LH and prolactin,6(10%) had elevated levels of FSH only with normal concentrations of LH and prolactin, 2(3.3%) had elevated levels of both FSH and LH with normal prolactin level, 23(38.3%) had elevated levels of prolactin(hyperprolactinaemia),19(31.7%) showed elevated levels of prolactin (hyperprolactinaemia) with normal levels of LH and FSH. The results obtained from the investigation were compared with standard values which are as follows: 0.5-15iu/l for LH, 0.2-10iu/l for FSH and 0-18.8ng/l for prolactin. The result showed a higher incidence (38.3%) of hyperprolactinaemia which has been identified by several other researchers as one of the major causes of female infertility .From this result, as corroborated by various other researchers, hormonal evaluation is important in the diagnosis and treatment of infertility as abnormalities in hormone concentrations may be the cause of infertility in some infertile women.