Antagonism of Bacillus spp. against Xanthomonas campestris pv. campestris

Leila Monteiro, Rosa de Lima Ramos Mariano, Ana Maria Souto-Maior
2005 Brazilian Archives of Biology and Technology  
The antagonism of eight Bacillus isolates was investigated against nine strains of Xanthomonas campestris pv. campestris (causal agent of crucifers black rot) to assess the role of lipopeptides in this process. Antimicrobial and hemolytic (surfactant) activity tests were performed in vitro using agar diffusion methods. Antibiosis and hemolysis were positive for four Bacillus isolates against all X. campestris pv. campestris strains. The correlation observed between antimicrobial and hemolytic
more » ... ial and hemolytic activities indicated that lipopeptides were involved in the antibiosis mechanism of the studied antagonists. Fermentation studies were carried out with the isolates that showed highest antimicrobial and hemolytic activities, to follow up growth and production of bioactive and surfactant compounds. Production of bioactive and surfactant compounds was observed during the late growth phase of the Bacillus isolates.
doi:10.1590/s1516-89132005000100004 fatcat:mr2wjpdu5bfnvct5ufv66mypim