Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum

Eliane P. Cervelatti, Monica S. Ferreira-Nozawa, Roseli Aquino-Ferreira, Ana Lúcia Fachin, Nilce M. Martinez-Rossi
2004 Genetics and Molecular Biology  
The electrophoretic karyotype of the dermatophyte Trichophyton rubrum was established using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Five chromosomal bands of approximately 3.0 to 5.8 megabase pairs (Mbp) each were observed and together indicated that 22.05 Mbp of the total genome are organized as chromosomal macromolecules. In addition to establishing the number and size of T. rubrum chromosomes, these results open perspectives for the construction of
more » ... ion of chromosome-specific libraries and for the physical mapping of genes of interest, thus permitting future gene linkage studies in this pathogen. A detailed understanding of the karyotype and genomic organization of T. rubrum should contribute to further genetic, taxonomic and epidemiological studies of this dermatophyte.
doi:10.1590/s1415-47572004000100016 fatcat:xel7vg54n5dddgn67eqqrekwki