PM2.5 exposure induced renal injury via the activation of the autophagic pathway in the rat and HK-2 cell

Xiaoliu Huang, Zhitong Zhou, Xinwen Liu, Jue Li, Lijuan Zhang
2020 Environmental Sciences Europe  
Exposure to airborne fine particulate matter (PM 2.5 ) has been declared to be harmful to human kidney. However, whether activation of the autophagic pathway plays key roles in the nephrotoxicity caused by PM 2.5 exposure is still poorly understood. The aim of this study was to explore the mechanism of kidney damage after PM 2.5 exposure in vivo and in vitro. Results: In the present study, statistically significant alterations in water intake, urine flow rate and mean blood pressure were
more » ... ressure were observed between the concentrated PM 2.5 (PM 2.5 ) group and the filtered air (FA) group. Exposed animals showed severe edema of renal tubular epithelial cells, capillary congestion, reduction of the glomerular urinary space and early pro-fibrotic state. Moreover, significant increases in the levels of early kidney damage markers were observed in the exposed rats and these animals exhibited more apoptosis rate in kidney cells. In addition, PM 2.5 exposure activated the autophagic pathway, as evidenced by LC3-I to LC3-II conversion, activation of P62 and beclin-1. All of these effects are in concurrence with the presence of more autophagosomes both in vivo and in vitro after PM 2.5 exposure. Conclusions: Taken together, our findings indicated that PM 2.5 induced renal function impairment via the activation of the autophagic pathway in renal tubular epithelial cells.
doi:10.1186/s12302-020-00378-7 fatcat:5gaqgtjo5ffjdoyx5cag46ksfu