Determination of Cell Wall Protein from Selected Feedstuffs and its Relationship with Ruminal Protein Digestibility in Vitro

A. Jayanegara, S. P. Dewi, N. Laylli, E. B. Laconi, Nahrowi Nahrowi, M. Ridla
2016 Media Peternakan  
This study was aimed to analyze neutral detergent insoluble crude protein (NDICP) and acid detergent insoluble crude protein (ADICP) contents of various commonly used forage and concentrate feedstuffs in Indonesia. A number of forages and concentrates, i.e. gliricidia, trichantera, indigofera, calliandra, papaya leaves, cassava leaves, leucaena, rapeseed meal, corn gluten feed, soybean meal, copra meal, palm kernel meal, fish and bone meal and wheat bran were subjected to proximate analysis,
more » ... ximate analysis, cell wall nitrogen determination and in vitro rumen fermentation evaluation. Chemical composition analysis was done in duplicate. The in vitro incubation was conducted in 14 treatments and 3 replicates by following a randomized complete block design. Variables measured after the incubation were total volatile fatty acid (VFA), ammonia, dry matter digestibility (DMD), organic matter digestibility (OMD) and crude protein digestibility (CPD). Results revealed that among the forages, cassava leaf had the highest proportion of NDICP while corn gluten feed was the highest among the concentrates. Different from that of NDICP, the highest proportion of ADICP was obtained in papaya leaf and copra meal for the forages and concentrates, respectively. Higher proportion of NDICP tended to decrease CPD (P<0.1). Although higher ADICP apparently tended to decrease CPD as well, the relationship was insignificant due to the higher variation of the dependent variable. It was concluded that cell wall protein proportion in feed may be used as an indicator to determine the quality of protein and its utilization in the rumen. ABSTRAK Penelitian ini bertujuan untuk menganalisis kandungan neutral detergent insoluble crude protein (NDICP) dan acid detergent insoluble crude protein (ADICP) pada sejumlah bahan pakan hijauan dan konsentrat yang umum digunakan di Indonesia. Bahan pakan berupa glirisidia, trichantera, indigofera, kaliandra, daun pepaya, daun singkong, lamtoro, bungkil rapeseed, corn gluten feed, bungkil kedelai, bungkil kelapa, bungkil inti sawit, tepung ikan dan tulang, dan dedak gandum dianalisis kandungan proksimat, nitrogen dinding sel, dan diinkubasi pada sistem fermentasi rumen secara in vitro. Analisis komposisi kimia dilakukan dalam 2 ulangan. Inkubasi in vitro dilakukan dalam 14 perlakuan dan 3 ulangan berdasarkan rancangan acak kelompok. Peubah yang diamati setelah inkubasi adalah total volatile fatty acid (VFA), amonia, kecernaan bahan kering (KBK), kecernaan bahan organik (KBO), dan kecernaan protein kasar (KPK). Hasil menunjukkan bahwa daun singkong mengandung NDICP tertinggi di antara hijauan, sedangkan corn gluten feed tertinggi di antara konsentrat. Proporsi ADICP tertinggi terdapat pada daun pepaya untuk hijauan dan bungkil kelapa untuk konsentrat. Proporsi NDICP yang semakin tinggi cenderung menurunkan KPK (P<0,1). Meskipun semakin tinggi ADICP juga cenderung menurunkan KPK, hubungannya relatif lemah dikarenakan tingginya variasi pada data KPK. Disimpulkan bahwa kandungan protein dinding sel pakan dapat digunakan sebagai indikator kualitas protein pakan dan penggunaannya di dalam rumen. Kata kunci: protein dinding sel, NDICP, ADICP, rumen, kecernaan
doi:10.5398/medpet.2016.39.2.134 fatcat:orh4k6w3rfff3iteebeiswuv34