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Citrus hystrix DC. locally known as shauk-nu belonging to Rutaceae family has been chosen to investigate some chemical analysis and antimicrobial activity on its fresh fruit peel. Phytochemical investigation reveals the presence of alkaloids, α-amino acids, carbohydrates, flavonoids, glycoside, phenolic compounds, reducing sugars, saponins, steroids, tannins and terpenoids. However, cyanogenic glycosides were absent by using the standard methods. In addition, 5.6 % of the essential oil contentdoi:10.5281/zenodo.3923280 fatcat:jbkty42jvzhdpncg7qqcz3syja