SCREENING ANTIBACTERIAL AND ANTIFUNGAL ACTIVITIES AND EVALUATION OF THE EXHAUSTIVE EXTRACTIONS YIELDS FOR VERBASCUM SINUATUM L
International Journal of Research in Ayurveda and Pharmacy
This study aimed to evaluate antibacterial and antifungal activities and exhaustive extraction yields of the aqueous and organic extracts of Verbascum sinuatum L., against possible human pathogens, which are the fungus Candida albicans, gram positive bacteria Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis and the gram negative bacteria Eschrichia coli and Pseudomonas aeruginosa. Well diffusion method was used in screening of antimicrobial activity for the plant extracts,
... e plant extracts, in which the diameters of inhibition zones were measured and compared to a positive control. Serial dilution method was used for measuring the minimum inhibition concentrations for each microorganism. In well diffusion method, the plant's aqueous extract has antimicrobial activity to all the tested organisms except Pseudomonas aeruginosa and Candida albicans, with variable diameters of inhibition zone. The percent inhibition compared to the positive control imipenem was 39.13 % for Staphylococcus aureus, 37.5 % for Staphylococcus epidermidis, 30.55 % for Eschrichia coli and the least 30.43 % for Bacillus subtilis. The organic extract exhibited inhibition activity 26.08 % against Bacillus subtilis and 50 % against Eschrichia coli. In the serial dilution method, the aqueous extract exhibited inhibition for all the test microorganisms. At initial concentration of 20 mg/ml, the lowest MIC value was for Staphylococcus aureus 1.28 µg/ml, and highest for Staphylococcus epidermidis 4000 µg/ml. The MIC values for Pseudomonas aeruginosa 160 µg/ml, 800 µg/ml for Bacillus subtilis, 800 µg/ml for Eschrichia coli and 32 µg/ml for Candida albicans respectively. This study showed that V. sinuatum extract has a broad spectrum activity against gram positive and gram negative bacteria, as well as anti candidal activity.