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PAA-SDS Gel Electrophoresis PAA-SDS gels (15%) were prepared according to Laemmly (6) and run at 20 mA for 1.5 h. After electrophoresis, the gels were stained with Coomassie blue R250. ABSTRACT A new approach for improving and scaling up the purification of recombinant human interferon gamma (hIFNγ) expressed in Escherichia coli and accumulated in the form of inclusion bodies was investigated. The new strategy involves denaturation of the inclusion bodies in 7.4M guanidine hydrochloride,doi:10.1080/13102818.2009.10817621 fatcat:mmejotmaanhzrolsoj6i65ufxu