A New Approach for Purification of Recombinant Human Interferon Gamma Expressed inEscherichia Coli

S. Petrov, E. Ivanova, D. Chakarova, V. Posheva, M. Redzheb, G. Nacheva, I. Ivanov
2009 Biotechnology & Biotechnological Equipment  
PAA-SDS Gel Electrophoresis PAA-SDS gels (15%) were prepared according to Laemmly (6) and run at 20 mA for 1.5 h. After electrophoresis, the gels were stained with Coomassie blue R250. ABSTRACT A new approach for improving and scaling up the purification of recombinant human interferon gamma (hIFNγ) expressed in Escherichia coli and accumulated in the form of inclusion bodies was investigated. The new strategy involves denaturation of the inclusion bodies in 7.4M guanidine hydrochloride,
more » ... drochloride, loading on a hydrophobic (Octyl-Sepharose) column and elution with urea/ammonium chloride. The method is fast, simple and low in cost. For maximum recovery of the hIFNγ biological activity, an optimization of the refolding step is proposed.
doi:10.1080/13102818.2009.10817621 fatcat:mmejotmaanhzrolsoj6i65ufxu