Optimization of the Reaction Conditions for the Peroxyoxalate Chemiluminescence Detection System of Fluorescent Compounds in a High-Performance Liquid Chromatography

Ryoya GOHDA, Kohsuke KIMOTO, Tomofumi SANTA, Takeshi FUKUSHIMA, Hiroshi HOMMA, Kazuhiro IMAI
1996 Analytical Sciences  
Using an instrument of automatic fluid injection (batch method), we optimized such reaction conditions as pH, concentration and kinds of buffer for the peroxyoxalate chemiluminscence (PO-CL) detection of fluorescent compounds for high-performance liquid chromatography (HPLC). The results were compared with those obtained by HPLC. Consequently, the usefulness of the batch method for investigating the reaction conditions for PO-CL detection in HPLC was demonstrated. With the optimized condition
more » ... timized condition using a phosphate buffer for the eluent in HPLC, the detection limits for Dns-Val and dipyridamole were 70 amol and 20 amol on column (S/N=2), respectively.
doi:10.2116/analsci.12.713 fatcat:63emnb7ff5hypdhmewzfeifyyy