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The restriction endonuclease BliAI, an isoschizomer of ClaI, which recognizes the sequence 5´-AT↓CGAT -3´, was purified from a natural isolate identified as Bacillus licheniformis. The restriction endonuclease was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. The restriction endonuclease is active at 37ºC and over a wide range of pH and salt concentration. The molecular weight of the purified restriction enzyme is consistent with a valuedoi:10.1590/s1517-83822006000400027 fatcat:gbyufz5sczhtbg3nhapnloxwom