Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis

Jocelei Maria Chies, Ana Christina de Oliveira Dias, Hélio Mauro Moreira Maia, Luis Paulo da Silva Braga, Spartaco Astolfi-Filho
2006 Brazilian Journal of Microbiology  
The restriction endonuclease BliAI, an isoschizomer of ClaI, which recognizes the sequence 5´-AT↓CGAT -3´, was purified from a natural isolate identified as Bacillus licheniformis. The restriction endonuclease was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. The restriction endonuclease is active at 37ºC and over a wide range of pH and salt concentration. The molecular weight of the purified restriction enzyme is consistent with a value
more » ... tent with a value of 39 kDa.
doi:10.1590/s1517-83822006000400027 fatcat:gbyufz5sczhtbg3nhapnloxwom