THE PRACTICAL APPLICATION OF THE RESULTS OF VITAMIN STUDIES
Journal of the American Medical Association (JAMA)
the appetite of their animals. These substances need be present in only minute quantity, but their presence is essential. It is this relation to appetite and food intake that prompts this report. In the course of certain studies on bacterial nutrition it became of interest, at one time, to test the basal ingredients of culture medium of organic origin, i. e., peptone and beef extract, for their vitamin content. This research was especially indicated, since the litera¬ ture is not clear on this
... not clear on this point. Bayliss,7 in his discus¬ sion of the growth promoting principle "Bios," says : "It is present in Liebig's meat extract and commercial peptone." Funk 8 reports the presence of vitamin in meat juice and meat extract, and Osborne and Mendel4 as well found "a small amount of the water soluble food hormone" in meat extract. EXPERIMENTAL METHODS USED The tests were carried out on young rats, weighing from 50 to 75 gm., which were failing to grow on a diet known to be adequate to promote growth at a normal rate when sufficient vitamin was supplied. The food mixture consisted of casein, 18 per cent.; sugar, 17; starch 52.5 ; butter fat, 10, and salt mixture, 2.5.