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Optimization of antimicrobial conditions and isolation of antimicrobial substances from Bacillus amyloliquefaciens X8
Purpose This study sought to employ the response surface methodology (RSM) to investigate factors that affect Bacillus amyloliquefaciens X8 bacteriostasis. Methods Design Expert 8.0.6 was used to perform multiple regression fitting and create antimicrobial mathematical models to optimize the antimicrobial effect of Bacillus amyloliquefaciens X8. A Shodex Protein KW-2004 gel column and a C18 column coupled with a high-performance liquid chromatography (HPLC) system were used to separate anddoi:10.21203/rs.3.rs-1477177/v1 fatcat:scruyhoqdfevhozzks2f4ecnve