Skin extracellular matrix components accelerate the regenerative potential of Lin− cells

Giedrė Ramanauskaitė, Dovilė Žalalytė, Vytautas Kašėta, Aida Vaitkuvienė, Lilija Kalėdienė, Genė Biziulevičienė
2014 Open Life Sciences  
AbstractDue to their unique properties, bone marrow-derived Lin− cells can be used to regenerate damaged tissues, including skin. The objective of our study was to determine the influence of the skin tissue-specific microenvironment on mouse Lin− cell proliferation and migration in vitro. Cells were analyzed for the expression of stem/progenitor surface markers by flow cytometry. Proliferation of MACS-purified cells in 3D cultures was investigated by WST-8 assay. Lin− cell migration was
more » ... gration was evaluated by in vitro scratch assay. The results obtained show that basement membrane matrix is more effective for Lin− cell proliferation in vitro. However, type I collagen matrix better enhances the re-epithelization process, that depends on the cell migratory properties. These studies are important for preparing cells to be used in transplantation.
doi:10.2478/s11535-013-0283-9 fatcat:i6o33vajcvf6njeya6362e4mj4