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Three experiments were conducted to elucidate the alleviation effects of Mycofix plus 3.0 on Newcastle antibody formation during aflatoxicosis in broiler chickens. Three levels of Mycofix (0.05%, 0.15%, and 0.25%) and aflatoxin (2.5ppm, 3.5ppm, and 5ppm) were used. Chickens were vaccinated at 8 and 18 days of age. Enzyme-linked immunosorbent assay and Haemagglutination inhibition tests were employed for determination Newcastle antibody titers at 28 days. The results showed that, Mycofix , anddoi:10.33899/ijvs.2008.5675 fatcat:q2kcy3dqtrdvdgiligqzxc6mqy