Advancing transcriptome platforms

Shuobo Shi, Shuobo Shi
2011 Nature Precedings  
During the last decade of years, remarkable technological innovations have emerged that allow the direct or indirect determination of transcriptome at unprecedented scale and speed. Studies using these methods have already altered our view of the extent and complexity of transcript profiling, which has advanced from one-gene-at-a-time to a holistic view of the genome. Here, we outline the major technical advances in transcriptome characterization, including the most popular used
more » ... sed platform, the well accepted tag-based sequencing platform, and the recently developed RNA-Seq (RNA sequencing) based platform. Especially, the nextgeneration technologies make a revolution in assessing the entire transcriptome via 1
doi:10.1038/npre.2011.5894 fatcat:3fyzpnkd5rhqrf4jjq5eufe6ey