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Field evaluation of a Pan-Lassa rapid diagnostic test during the 2018 Nigerian Lassa fever outbreak

Matthew L. Boisen, Eghosa Uyigue, John Aiyepada, Katherine J. Siddle, Lisa Oestereich, Diana K. S. Nelson, Duane J. Bush, Megan M. Rowland, Megan L. Heinrich, Philomena Eromon, Adeyemi T. Kayode, Ikponmwosa Odia (+32 others)
2020 Scientific Reports  

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Lassa virus (LASV) is the causative agent of Lassa fever (LF), an often-fatal hemorrhagic disease. LF is endemic in Nigeria, Sierra Leone and other West African countries. Diagnosis of LASV infection is challenged by the genetic diversity of the virus, which is greatest in Nigeria. The ReLASV Pan-Lassa Antigen Rapid Test (Pan-Lassa RDT) is a point-of-care, in vitro diagnostic test that utilizes a mixture of polyclonal antibodies raised against recombinant nucleoproteins of representative
more » ... from the three most prevalent LASV lineages (II, III and IV). We compared the performance of the Pan-LASV RDT to available quantitative PCR (qPCR) assays during the 2018 LF outbreak in Nigeria. For patients with acute LF (RDT positive, IgG/IgM negative) during initial screening, RDT performance was 83.3% sensitivity and 92.8% specificity when compared to composite results of two qPCR assays. 100% of samples that gave Ct values below 22 on both qPCR assays were positive on the Pan-Lassa RDT. There were significantly elevated case fatality rates and elevated liver transaminase levels in subjects whose samples were RDT positive compared to RDT negative.
doi:10.1038/s41598-020-65736-0 pmid:32457420 pmcid:PMC7250850 fatcat:pb2fdocl55aulkixe3qativ6cu
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Matthew L. Boisen, Eghosa Uyigue, John Aiyepada, Katherine J. Siddle, Lisa Oestereich, Diana K. S. Nelson, Duane J. Bush, Megan M. Rowland, Megan L. Heinrich, Philomena Eromon, Adeyemi T. Kayode, Ikponmwosa Odia, Donatus I. Adomeh, Ekene B. Muoebonam, Patience Akhilomen, Grace Okonofua, Blessing Osiemi, Omigie Omoregie, Michael Airende, Jacqueline Agbukor, Solomon Ehikhametalor, Chris Okafi Aire, Sophie Duraffour, Meike Pahlmann, Wiebke Böhm, Kayla G. Barnes, Samar Mehta, Mambu Momoh, John Demby Sandi, Augustine Goba, Onikepe A. Folarin, Ephraim Ogbaini-Emovan, Danny A. Asogun, Ekaete A. Tobin, George O. Akpede, Sylvanus A. Okogbenin, Peter O. Okokhere, Donald S. Grant, John S. Schieffelin, Pardis C. Sabeti, Stephan Günther, Christian T. Happi, Luis M. Branco, Robert F. Garry. "Field evaluation of a Pan-Lassa rapid diagnostic test during the 2018 Nigerian Lassa fever outbreak." Scientific Reports 10.1 (2020) 8724