Pectobacterium atrosepticum Biosensor for Monitoring Blackleg and Soft Rot Disease of Potato

Mahdis Hashemi Tameh, Elisabetta Primiceri, Maria Serena Chiriacò, Palmiro Poltronieri, Masoud Bahar, Giuseppe Maruccio
2020 Biosensors  
Pectobacterium atrosepticum (Pba) is a quarantine and threatening phytopathogen known as the causal agent of blackleg and soft rot disease of potatoes in many areas. Its early detection is then important to have healthy potato tubers and reduce economic losses. Today, conventional methods such as enzyme-linked immunosorbent-assay (ELISA) and polymerase chain reaction (PCR) are typically used for Pba detection, but they are expensive and time-consuming. Here we report on the optimization of an
more » ... ptimization of an alternative approach based on an electrochemical impedance immunosensor combining a microfluidic module and a microelectrodes array, and having advantages in terms of low cost, ease of use and portability. For validation and for assessing its performance, the lab-on-chip platform has been compared with two standard methods (ELISA and PCR).
doi:10.3390/bios10060064 pmid:32549369 fatcat:2ikzckm655cgjcwsy7tubk2gfq