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A method employing high-performance liquid chromatography with electrochemical detection (LC-ED) has been successfully developed for the determination of uric acid in human salvia. The optimal chromatographic conditions were found to comprise a mobile phase containing 5 % methanol, 95 % 50 mM trichloroacetic acid, adjusted to pH 2.7, in conjunction with a Hypersil C 18 250 mm x 4.6 mm column at a flow rate of 0.6 mL min -1 . Hydrodynamic voltammetric studies were undertaken to optimize thedoi:10.15436/2476-1869.17.1280 fatcat:ixv43syuhrabhderiig3nncxjy