The Determination of Uric Acid in Human Saliva by Liquid Chromatography with Electrochemical Detection

Kevin Honeychurch
2017 Journal of Analytical Bioanalytical and Separation Techniques  
A method employing high-performance liquid chromatography with electrochemical detection (LC-ED) has been successfully developed for the determination of uric acid in human salvia. The optimal chromatographic conditions were found to comprise a mobile phase containing 5 % methanol, 95 % 50 mM trichloroacetic acid, adjusted to pH 2.7, in conjunction with a Hypersil C 18 250 mm x 4.6 mm column at a flow rate of 0.6 mL min -1 . Hydrodynamic voltammetric studies were undertaken to optimize the
more » ... o optimize the operating potentials required for electrochemical detection. It was found that an applied potential of +1.2 V was optimum for the determination of uric acid. The proposed method was evaluated by carrying out replicate uric acid determinations on spiked and unspiked human saliva samples (%CV = 8.8 %). The data suggest that the method holds promise for clinical applications.
doi:10.15436/2476-1869.17.1280 fatcat:ixv43syuhrabhderiig3nncxjy