An Ultrahighly Sensitive Electrochemical Biosensor for Cytochrome c with Surface Molecular Imprinting Based on Hybrid Nanomaterials

Qin Wang, College of Chemistry and Chemical Engineering, Key Lab of Bioelectrochemistry and Environmental Analysis of Gansu Province, Northwest Normal University, Lanzhou 730070, PR China
2020 International Journal of Electrochemical Science  
A novel ultrahighly sensitive 3D structural electrochemical imprinted biomacromolecular sensor for Cyt c was fabricated based on hybrid polyaniline nanotube/carboxylated multi-walled carbon nanotubes (PANTs-MWCNTs) composites by surface molecular imprinting method on the surface of glassy carbon electrode (GCE). The hybrid nanomaterials can enhance imprinting efficiency. The fabrication and electrochemical behavior of the prepared imprinted biosensor were studied by electrochemical techniques,
more » ... emical techniques, scanning electron microscopy (SEM) and Fourier transform infrared spectra (FTIR). Under the optimal conditions, the detection of Cyt c with the imprinted sensor exhibited a wide linear range from 1.0×10 -14 to 1.0×10 -6 mg/mL with lower detection limit of 7.62 ×10 -16 mg/mL. The developed procedure has been successfully used in assay of Cyt c in real samples.
doi:10.20964/2020.01.25 fatcat:yzdfnztiqzg23o33u2a6bk7ipq