Fluorometric determination of sulfites in rainwater

Kazuaki AKASAKA, Tateo SUZUKI, Hiroshi OHRUI, Hiroshi MEGURO
1986 Analytical Sciences  
A simple and specific fluorometric determination of sulfites in rainwater was developed. Rainwater was collected in the field in a trapping buffer containing EDTA (pH 10.0), which effectively prevented the loss of sulfites by oxidation. Then its aliquot was labeled with N-(9-acridinyl)maleimide (NAM) and the concentration was determined on a high performance liquid chromatograph equipped with a fluorometer. The concentration range of the determination was about 1.0-100 pmol on column. The
more » ... ination required less than five ml of rainwater. The recovery of the added sulfite was almost quantitative.
doi:10.2116/analsci.2.443 fatcat:ob6wu5fvnrbkzpn5ducy23pw6e