Purification and antigenic specificity of alpha protein (Yoneda and Fukui) from Mycobacterium tuberculosis and Mycobacterium intracellulare

H Tasaka, K Kiyotani, Y Matsuo
1983 Hiroshima journal of medical sciences  
Alpha protein antigen isolated from culture filtrate of Mycobacterium tuberculosis by Yoneda and Fukui (1961) was a cross-reacting material among mycobacteria. Purified alpha protein of M tuberculosis obtained by a series of procedures with gel filtration, ion-exchange chromatographies, and chromatofocusing. Alpha protein of Mycobacterium intracellulare was purified by an affinity chromatography on anti M tuberculosis-alpha serum conjugated sepharose. Alpha antigen of M tuberculosis possessed
more » ... culosis possessed specific antigenic determinants for both M tuberculosis and M bovis, and so did alpha antigen of M intracellulare for both M intracellulare and M avium. Extracts from M kansasii, M. scrofulaceum, M gordonae, M xenopi and M gastri showed alpha antigenicity somewhat different from those of M tuberculosis and M intracellulare. Alpha antigen was not detected in extracts from M nonchromogenicum, M terrae and M triviale.
pmid:6190783 fatcat:wpi3vk43nnhzxooajleen7ky2m