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An Acetocarmine Staining Procedure for Chromosome Banding Studies of Immature Pollen in Triticeae
J. Agr. Sci. Tech
Studies of postmeiotic chromosomes have been impeded by the thick exine of immature pollen grains. Chromosome staining of immature and adult pollen grains through conventional acetocarmine procedure is tedious and often leads to unsatisfactory results. An acetocarmine stain, used in conjugation with Carnoy's solution as fixative for 72 hrs or until the chlorophyll-containing tissues of spike became colorless, were successfully used to stain chromosome bands and nucleoli of the immature pollenfatcat:sjix4nubjfgedbgxg5amx4jx44