Ileal Transcriptome Profiles of Japanese Quail Divergent in Phosphorus Utilization

Michael Oster, Henry Reyer, Nares Trakooljul, Frank M. Weber, Lu Xi, Eduard Muráni, Siriluck Ponsuksili, Markus Rodehutscord, Jörn Bennewitz, Klaus Wimmers
2020 International Journal of Molecular Sciences  
Phosphorus (P) is an essential component for all living beings. Low P diets prompt phenotypic and molecular adaptations to maintain P homeostasis and increase P utilization (PU). Knowledge of the molecular mechanisms of PU is needed to enable targeted approaches to improve PU efficiency and thus lower P excretion in animal husbandry. In a previous population study, Japanese quail were subjected to a low P diet lacking mineral P and exogenous phytase. Individual PU was determined based on total
more » ... ned based on total P intake and excretion. A subset of 20 extreme siblings discordant for PU was selected to retrieve gene expression patterns of ileum (n = 10 per PU group). Sequencing reads have been successfully mapped to the current Coturnix japonica reference genome with an average mapping rate of 86%. In total, 640 genes were found to be differentially abundant between the low and high PU groups (false discovery rate ≤ 0.05). Transcriptional patterns suggest a link between improved PU and mitochondrial energy metabolism, accelerated cell proliferation of enterocytes, and gut integrity. In assessing indicators of the efficient use of macro- and micronutrients, further research on turnover and proliferation rates of intestinal cells could provide an approach to improve P efficiency in poultry species.
doi:10.3390/ijms21082762 pmid:32316159 fatcat:xu33vvbh5vgx3pvmhr5k23vu2a