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Owing to non-degradable waste plastics caused serious environmental pollution, development of natural polymeric materials has become an important topic. Herein, multilayer tunicate cellulose films (MTCFs) were fabricated by a top-down approach, where the alkali treated mantles of tunicate were layer-by-layer hot-pressed. Strong hydrogen bonds that formed between tunicate cellulose layers resulted in good interfacial compatibility of multilayer films. The as-prepared MTCFs exhibited improveddoi:10.21203/rs.3.rs-243240/v1 fatcat:isiv4kdp5nhu7mwutp5jqlhpvu