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Seven Bulgarian strains of Erwinia amylovora isolated from chokeberry and strawberry, previously identified by single PCR amplification of a specific region of plasmid pEA29 and genome ams-region were subjected to multiplex PCR using two pairs of primers -pEA29A/pEA29B and AJ245/AJ246. These primers allowed the identification of the strains in one-step PCR simultaneously in two genetic loci. The conditions of the multiplex PCR were optimized, and any nonspecific reactions with other related,doi:10.1080/13102818.2006.10817375 fatcat:nngz2a55qzfp3ky26vjlapxu5q