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Endophytes are mutualistic symbionts of healthy plants known for production of wide range of bioactive compounds with a broad spectrum of activity. In the present study, endophytic Aspergillus fumigatus was isolated from Justicia beddomei. The bioactive compounds were extracted with ethyl acetate by solvent extraction procedure. Fraction IV obtained by partial purification with a mixture of hexane and ethyl acetate in silica gel column demonstrated cytotoxicity on A549 and L6 cell lines. The ICdoi:10.13005/bbra/1340 fatcat:xkgkk5syibbmbcekdlkkk43riy