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Reliability of T7-Based mRNA Linear Amplification Validated by Gene Expression Analysis of Human Kidney Cells Using cDNA Microarrays
Nephron Experimental Nephrology
Genome wide gene expression analysis by cDNA microarrays is often limited by minute amounts of starting RNA. We therefore tested an optimized linear RNA amplification protocol using the RiboAmp ® amplification kit in the setting of cDNA microarrays. We isolated mRNA from a human kidney cell line (HK-2; ATCC) and from Universal Human Reference RNA (STR; Stratagene). After performing one and two rounds of linear RNA amplification, respectively, the amplified RNAs were co-hybridized to cDNAdoi:10.1159/000078642 pmid:15292679 fatcat:y5tozepvqneu7dxrwunhruuob4