Intrapopulational genetic differentiation in Anopheles (N.) darlingi Root, 1926 (Diptera: Culicidae) in the amazon region

Joselita Maria Mendes dos Santos, Jorge Arturo Lobo, Wanderli Pedro Tadei, Eucleia P. Betioli Contel
1999 Genetics and Molecular Biology  
Four natural Amazonian Anopheles darlingi populations were analyzed for 19 loci, 12 of which showed variation, i.e., 68.4% of polymorphic loci. The Ariquemes, Rondônia, population was the most variable, with a large number of alleles per locus (2.26 ± 0.27) and high polymorphism (P = 63.15). The highest values of observed and expected intralocus heterozygosity were observed in the Manaus, Amazonas, population (Ho = 0.432 ± 0.11; He = 0.375 ± 0.08), and the lowest in the Cachoeira Porteira,
more » ... eira Porteira, Pará, population (Ho = 0.236 ± 0.09; He = 0.290 ± 0.11). Wright's F-statistic revealed disequilibrium caused by an excess of homozygotes, as shown by the Fis > Fst values (Fis = 0.083 > 0.026) reflecting intrapopulational differentiation. The four populations studied were genetically similar, as indicated by distance and similarity values. Chromosomal variation of Amazon population also did not indicate geographical differentiation, and populations in the central region of the Amazon Basin showed high polymorphism in relation to the marginal populations, which were mainly monomorphic.
doi:10.1590/s1415-47571999000300007 fatcat:xfq2tvxjirg77isdbni4mr4ppq