Detection of Enterotoxin Types Produce by Coagulase Positive Staphylococcus species Isolated from Mastitis in Dairy Cows in Sulaimaniyah Region
Applied Science Reports
This study included 250 dairy cows (985 quarters) selected randomly from six selected dairy herds and also from individual cows from areas around Sulaimaniyah region including Bazyan, Tanjarwo, Arbit, Peramagrwn, Kanakawa and Tagaran during March to August, 2007. It was found that the prevalence of bovine mastitis was 80 % (200) at cow level. Out of 200 cows (785 quarters), it was found that the incidence of clinical mastitis was 26 % cows (65), 25.4% quarters (250), while the incidence of
... e incidence of sub-clinical mastitis was 54 % cows (135), 54.3% quarters (535). According to manitol ferment, coagulase, oxidase, catalase, urease, and DNase tests of 200 cows (785 quarters), it was found that 246 isolates (31.3%) were coagulase positive staphylococci (CoPS). An Api-Staph system was used in assuring a diagnosis for these bacteria, which showed complete matching with traditional biochemical tests. Production of enterotoxin A, B, C and D was detected by a reverse passive latex agglutination test (RPLA). Among 246 CoPS isolates, 113 (45.9%) were found to be positive for production of one or more of enterotoxin types A, B and C. The production of enterotoxin types A, B, C, D, E, G, H, I and J were also tested by PCR technology. Among 246 CoPS isolates, 125 (50.8%) were found to be positive for production of one or more enterotoxin types A, B, C and E.