Studies on the Determination of the Minimal Inhibitory Concentration of 4,4'-diamino-diphenyl-sulphone (Dapsone, DDS) againstMycobacterium leprae

G. A. ELLARD, PATRICIA T. GAMMON, R. J. W. REES, M. F. R. WATERS
1971 Leprosy Review  
Plasma DDS concentrations were determined fluorometrically in mice fed continuously with 0.0 1% of the drug in their diet. They averaged 0.74 J.1.g per m!. (-2) Since it is known that the multiplication of My co. leprae in the mouse footpad system is inhibited by feeding 0.000 1% DDS but not by 0.0000 1% DDS in the diet, it was concluded that the MIC of DDS against My co. leprae must be less than 0.01 J.1.g per m!. .£3) The half-life of DDS in the mouse was determined after intraperitoneal
more » ... ntraperitoneal dosage and found to be 2.7 h after dosage with DDS at 10 mg per kg. and 3.7 h after 50 mJ per kg. {'4r DDS serum concentrations in patients who were being successfully treated witli I mg of DDS a day averaged 0.0 18 J.1.g per ml 3 h after daily dosage, and a similar proportion of the drug was excreted in the urine as was found with doses some 50 to 300 times as large. {'5 J )-The significance of these findings is discussed both in the relation to the treatment of leprosy with DDS, and to the use of the mouse footpad system in predicting the likely effi cacy of other potential antileprosy compounds.
doi:10.5935/0305-7518.19710013 fatcat:xiv4xyxk4zbkjnzodvqjyooiki