Comparative Studies of Invertase Enzyme from Low Cost Agricultural Waste Substrates By Saccharomyces CerevisiaeIsolated From Grape Juice

Jeyabharathi. S
2017 International Journal for Research in Applied Science and Engineering Technology  
The official name for invertase is β-fructofuranosidase (EC.3.2.1.26), which implies that the reaction catalyzed by the enzyme, is the hydrolysis of the terminal non-reducing β-fructofuranoside residues in β-fructofuranosides.In this present study screen the industrially important yeast Saccharomyces cerevisiaefrom grape juice sample using serial dilution and yeast peptone dextrose agar plating technique. The isolate was checked for the invertase enzyme production by using different low cost
more » ... fferent low cost agricultural waste substrates like Ricebran,Wheatbran,Ground nut cake flakes and Sucrose.Invertase was characterized by protein and carbohydrate estimations and enzyme assay. The partial purification of this enzyme was studied by ammonium sulphate precipitation, membrane Dialysis. The results showed in this study about 43.5 U/ml for Rice bran, 72.5 U/ml for Wheat bran, 63.3U/ml for Ground nut cake flakes and 83.5U/ml for Sucrose.
doi:10.22214/ijraset.2017.10023 fatcat:b56f6bhwjrhetfi5dczrcjwila