Dendropsophin 1, a novel antimicrobial peptide from the skin secretion of the endemic Colombian frog Dendropsophus columbianus

Luz Elena Triana-Vidal, Mariana Souza Castro, Osmindo Rodrigues Pires Júnior, Alice Cunha Morales Álvares, Sonia Maria De Freitas, Wagner Fontes, Jimmy Alexander Guerrero Vargas, Jorge Alberto Zúñiga-Baos, Isabel De Fátima Correia Batista, Philippe Grellier, Sébastien Charneau
2017 Figshare  
In efforts to find new antimicrobial peptides (AMPs), we studied the skin secretion of the endemic Colombian frog Dendropsophus columbianus belonging to a genus that has not been investigated previously. From HPLC-fractionated secretion, we identified one peptide with slightly antibacterial activity. Its peptide sequence showed no sequence similarity to current annotated peptides. We named this novel peptide dendropsophin 1 (Dc1). Afterward, two analogues were designed (Dc1.1 and Dc1.2) to
more » ... and Dc1.2) to improve the cationic and amphipathic features. Then, their antiproliferative and cytotoxic properties were evaluated against several pathogens including bacteria, fungi, protozoa and also mammalian cells. Dc1 and its two analogues exhibited moderate antibacterial activities and no hemolytic and cytotoxic effects on mammalian cells. Analogue Dc1.2 showed slightly improved antibacterial properties. Their secondary structures were characterised using CD spectroscopy and Dc1.2 displayed a higher α-helix content and thermal stability compared to Dc1 and Dc1.1 in hydrophobic experimental conditions.
doi:10.6084/m9.figshare.5155069 fatcat:gxhawtm7m5dxxktdagdmyuyfxi