Genome-Wide Identification of AP2/EREBP in Fragaria Vesca and Expression Pattern Analysis of The FvDREB Subfamily Under Drought Stress
Background: Drought is a common phenomenon worldwide. It is also one of the main abiotic factors that affect the growth and quality of strawberry. The dehydration-responsive element binding protein (DREB) members that belong to the APETALA2/ethylene-responsive element binding protein (AP2/EREBP) superfamily are unique transcription factors in plants, which play an important role in the abiotic stress response. Results: Here, a total of 119 AP2/EREBP genes were identified in F. vesca, and the
... . vesca, and the AP2/EREBP superfamily was divided into AP2, RAV, ERF, DREB, and soloist subfamilies, containing 18, 7, 61, 32, and 1 member(s), respectively. The DREB subfamily was further divided into six subgroups (A-1 to A-6) based on phylogenetic analysis. Gene structure, conserved motifs, chromosomal location, and synteny analysis were conducted to comprehensively investigate the gene characteristics of FvDREBs. Furthermore, transcriptome analysis revealed distinctive expression patterns among the FvDREB genes in strawberry plants exposed to drought stress. The expression of FvDREB6 of the A-2 subgroup was down-regulated in old leaves and up-regulated in young leaves in response to drought. Furthermore, qRT-PCR analysis found that FvDREB8 of the A-2 subgroup had the highest expression when plants were subjected to drought stress. Both analyses combined with promoter analysis suggested that FvDREB18 may play a critical role in regulating the expression of FvDREB1 and FvDREB2. Conclusions: Our findings provide new insights into the characteristics and potential functions of FvDREBs. And these FvDREB genes should be further studied as they appear to be excellent candidates for drought tolerance improvement of strawberry.