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For the determination of methylation levels in genomic regulatory DNA sequences a high-sensitive assay for detecting 5 0 methyl-cytosines (5 0 mC) in non-bisulfite-treated DNA has been established. The system is designed for the application of immunofluorescence using a monoclonal antibody that specifically recognizes 5 0 mC in single-stranded DNA hybridized to oligonucleotide microarrays. For assay readout an ultra-sensitive fluorescence scanner with submicrometer resolution was used. Todoi:10.1093/dnares/dsi024 pmid:16766511 fatcat:hzkvnglmb5gklhaozweciw4u3q