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1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) is a very useful agent to form amide bonds (peptide bonds) in an aqueous medium. A simple and fast detection system was developed using the reaction with pyridine and ethylenediamine in acidic aqueous solution and spectrophotometric flow injection analysis. The absorbances were measured at 400 nm and the reaction was accelerated at 40˚C. The calibration graph showed good linearity from 0 to 10% of EDC·HCl solutions: thedoi:10.2116/analsci.24.505 pmid:18403843 fatcat:l7gy655zljh6pl7b6pyqltcfwi