Activated Blood Coagulation Factor X (FXa) Induces Angiogenic Growth Factor Expression in Human Retinal Pigment Epithelial Cells

Margrit Hollborn, Leon Kohen, Cornelia Werschnik, Lore Tietz, Peter Wiedemann, Andreas Bringmann
2012 Investigative Ophthalmology and Visual Science  
PURPOSE. To determine the transcriptional regulation of the blood coagulation factor X (FX) in cultured human retinal pigment epithelial (RPE) cells, and whether the effects of FXa on the chemotaxis and expression of angiogenic growth factors are mediated by autocrine growth factor signaling. METHODS. Alterations in gene expression and secretion of growth factors were determined by real-time RT-PCR and ELISA, respectively. Cellular proliferation and chemotaxis were investigated with a
more » ... ed with a bromodeoxyuridine immunoassay and a Boyden chamber assay, respectively. From the
doi:10.1167/iovs.11-9214 pmid:22871831 fatcat:gb5eag45xjbr7gxf3guzpsyd2q