cDNA Cloning, Tissue Expression, and Chromosomal Assignment of a Mouse Gene, Encoding a 127 kDa UV-damaged DNA Binding Protein Which is Defective in XPE Cells

N. Seki
1999 DNA Research  
A subset of xeroderma pigmentosum (XP) group E cells lack a factor of the UV-damaged DNA binding activity. Both 127 kDa and 48 kDa proteins have been reported to be responsible for the binding activity. A cDNA for the 127 kDa UV-damaged DNA-binding protein (pl27-Ddbl) was isolated from a mouse fetal brain full length-enriched cDNA library, and an open reading frame of 1140 amino acids was identified. Reverse transcription-coupled polymerase chain reaction (RT-PCR) showed that mouse Ddbl
more » ... mouse Ddbl messenger is ubiquitously expressed in adult tissues as well as in embryo's. The gene was mapped to near the public locus D19Mit22 region of mouse chromosome 19.
doi:10.1093/dnares/6.5.319 pmid:10574459 fatcat:47tknpohmnafhb3stz3u4addoq