Evaluation of chemiluminescence method for the analysis of plasma homocysteine and comparison with HPLC method in children samples

Thais Moura Gascón, Fernanda Schindler, Claudia Giorgia Bronzatti de Oliveira, Fabiola Isabel Suano Souza, Sonia Hix, Roseli Oselka Sacardo Sarni, Ana Paula Arantes Bacan, Vania D'Almeida, Auro del Giglio, Fernando Luiz Affonso Fonseca
2010 Einstein (São Paulo)  
Objective: To compare the results for homocysteine concentration using chemiluminescence and HPLC methods in samples from school-age children. In addition, to determine the reference values for patients of this age group and assess the real prognostic value of homocysteine in healthy children. Methods: A prospective observational study was undertaken to determine plasma levels of homocysteine using two different assays, HPLC and chemiluminescence, in 185 samples from school-age children living
more » ... ge children living in Santo Andre, with no chronic or inflammatory diseases, and absence of pubertal development. Results: The results were presented in percentiles and reference values were determined within this age group (7-9 years old). Homocysteine concentration ranged from 2.0 to 9.9 µmol/l (r = 0.821 and p < 0.001). Conclusions: It was verified that chemiluminescence is comparable to HPLC when both techniques are used to detect homocysteine in school-age children. There is an important correlation between both methods, which allows investigation of this amino acid as a risk factor for heart diseases.
doi:10.1590/s1679-45082010ao1499 pmid:26760001 fatcat:lmpacat4cfbyhbvlt5xej54fty