High-Pressure Gel Loader for Capillary Array Electrophoresis Microchannel Plates

J.R. Scherer, B.M. Paegel, G.J. Wedemayer, C.A. Emrich, J. Lo, I.L. Medintz, R.A. Mathies
2001 BioTechniques  
Microfabricated capillary array electrophoresis ( µ CAE) microchannel plates are the next generation of bioanalytical separation devices. To fully exploit the capabilities of µ CAE devices, supporting technology such as robotic sample loading, gel loading, microplate washing, and data analysis must be developed. Here, we describe a device for loading gel into radial capillary array electrophoresis microplates and for plate washing and drying. The microplates are locked into a loading module,
more » ... loading module, and high-pressure helium is used to drive aqueous separation media or wash solutions into the microchannels through fixtures connected to the central anode reservoir. Microplates are rapidly (30 s to 5 min) loaded with separation media, such as 3%-4.8% linear polyacrylamide or 0.7% -3.0% hydroxyethyl cellulose, for electrophoresis. The effective and rapid gel-filling and plate-cleaning methods together with short electrophoretic analysis times (2-30 min) make µ CAE systems versatile and powerful nucleic acid analysis platforms. R e searchR e po r t
doi:10.2144/01315rr04 pmid:11730021 fatcat:4u2z2eaccvhhxnivg3ktg7ltkm