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Five new natural compounds (1–5) along with four known ones, involving dibenzo-α-pyrone derivatives, a benzo-γ-pyrone derivative and an amide-type compound were obtained from Alternaria alternata, an endophyte isolated from Paeonia lactiflora. The structures of these isolates were elucidated by intensive analysis of spectroscopic data including NMR, HRMS (ESI and EI), UV and IR spectra. Compounds (1–4) were evaluated for their cytotoxicities against five selected human tumourtumour cell linesdoi:10.6084/m9.figshare.8870498 fatcat:gsnskmicsjhmlmhi35mxuoopty