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We have isolated a thermotolerant yeast strain, called TR2, from banana leaves and characterized it as Saccharomyces cerevisiae by sequencing about 25 kb at random, including structural genes and gene promoters. In all cases, the sequence identity was higher than 98%, and gene synteny was conserved. The strain is a homothallic diploid strain containing two sets of S. cerevisiae chromosomes that grows efficiently in a complete medium up to 40-41 °C. We present a method allowing the isolation ofdoi:10.2306/scienceasia1513-1874.2008.34.147 fatcat:ler3nwvlsna5te4qwr6ybbbihy