Reload Purified Melittin and Lactoferrin on Perfluorooctyl Bromide Nanoparticles (PFOB-Nps) and Examine the Distribution of Particle Size, Zeta Potential and Confirmation of Their Accession on the Nanoparticles Via Tryptophan Fluorescence and Circular Dichroism (CD) and its Anti-Cancer Effects on Human Breast Cancer Cell Line MCF7

Hadis Keykanlu, Saeid Zibaei, Mehdi Ardjmand, Ali Safekordi
2016 Oriental Journal of Chemistry  
Acording to the prevalence of cancer in today's societies, it is too important to find new drugs for that treatment. Cationic antimicrobial peptides that are able to eliminate a wide range of bacteria, fungi, parasites, viruses and unicellular, are natural anti-cancer agents. In the present study, the effect of cationic peptides of bee venom Melittin (ML) and Lactoferrin (LF) of camel milk which was loaded on the Perfluorooctyl Bromide (PFOB) nanoparticles, were examined on MCF7 cells.
more » ... CF7 cells. Nanoparticles using oil in water emulsion was prepared by ultra-thoracic and ultrasonic. Then ML and LF were separately added to the nanoparticles then incorporation of them were confirmed by tryptophan fluorescence and circular dichroism (CD) spectra. The mean diameter of the nanoparticles did not change after addition of ML and LF (~100 nm), but as expected, the zeta potential of the nanoparticles with increasing of ML and LF concentrations to the preformed nanoparticles increased from -18.43(without ML and LF) to +21.61(LF 110.66µg/ml) and +20.93(ML 0.1µg/ml) and +23.75(LF 110.66µg/ml and ML 0.1µg/ml. By using of scanning electron microscopy (SEM), the morphology and structure of nanoparticles was investigated, homogeneity and uniformity during processing and loading of ML and LF was observed. Effectiveness of nanoparticles loaded on cancer cells was performed with MTT test. The results showed that ML and LF had a positive effect on cancer cell death. Percent of cell death was higher when ML and LF was loaded on nanoparticles. With increase of ML and LF concentration loading on nanoparticles, increases cancer cell death. Minitab software was used to analyze some data.
doi:10.13005/ojc/320631 fatcat:pehwqrpidnamnawkqua3mmr4d4