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One of the basic properties of confocal scanning laser microscopy (CSLM) is the possibility of high axial resolution in the case of fluorescent object% As a consequence 3D images of 30 objects can be obtained. In the previous papers of this series it has been demonstrated that an improvement of lateral resolution in CSLM (lateral super-resolution) can be obtained if, at each step of the scanning procedure, the full image is detected in the image plane and these data are inverted to estimate thedoi:10.1088/0266-5611/10/5/005 fatcat:bifukkt3lzgxrmbkqbwxyscvam