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specific RT-PCR products were not generated (lanes 3 and 12). The same results were obtained for the other six mRNAs investigated using S2-A2, S3-A3, S4-A4, S5-A5, S6-A6, and S7-A7 primer pairs (data not shown). Thus, the results presented in this article show that, depending on the reaction conditions (i.e., enzyme concentration, temperature, and buffer composition), RTases from different manufacturers demonstrate different specificity and background activity. Without preliminary selection ofdoi:10.2144/01301bm03 pmid:11196315 fatcat:ks5vjqya4nbhhfrhfszovqfw3i