Voltametria de Pulso Diferencial (VPD) em estado sólido de manchas de Cromatografia de Camada Delgada (CCD): um novo método de análise para fitoativos antioxidantes

Darlene Gonçalves, Renê O. Couto, Edemilson C. Conceição, Nathasha S. Reis, Eric S. Gil
2011 Química Nova  
Recebido em 17/3/10; aceito em 3/8/10; publicado na web em 30/11/10 SOLID STATE DIFFERENTIAL PULSE VOLTAMMETRY (DPV) FROM SPOTS OF THIN LAYER CHROMATOGRAPHY (TLC): A NEW METHOD FOR ANALYSIS OF ANTIOXIDANT PHYTOACTIVES. A new electroanalytical method coupling TLC-DPV in solid state was developed for quantitative determination of phytoantioxidants with medicinal purpose, e.g. rosmarinic acid (RA) in samples of phytopharmaceuticals, e.g. rosemary (Rosmarinus officinalis L.). The method showed to
more » ... method showed to be feasible, presenting linearity in concentrations ranging from 0.694 x 10 -3 to 9.526 x 10 -3 mol L -1 (r = 0.9945), good sensibility, selectivity, reproducibility, repeatability, agility and affordable cost. The concentrations of RA in different extracts of rosemary ranged from 0.05 to 0.52 (% w/w), presenting high recovery levels when compared to HPLC.
doi:10.1590/s0100-40422011000200028 fatcat:kfxftoxfhfdhjj4narqwx4rzu4