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The present research was undertaken to isolate and characterize petroleum hydrocarbon degrading microbes from ship-breaking yards at Vatiary and Kumira coast in Chittagong. Twenty different petroleum hydrocarbon contaminated composite samples were collected and the total bacterial count was found to vary between 2.7×10 3 cfu/ gm and 1.77×10 7 cfu/ gm. Ten isolates were finally selected through secondary screening by Bushnell-Hass mineral salt medium using kerosene, diesel and engine oil asdoi:10.3329/bjm.v27i1.9161 fatcat:6fhnou4mindsjfpb6njnkuow6e