Isolation and Chemiluminescent Properties of Ferret (Mustela putorius furo) Polymorphonuclear Cells

Makoto NAKATA, Takuya ITOU, Takeo SAKAI
2007 Journal of Veterinary Medical Science  
Ferret polymorphonuclear cells (PMNs) and peripheral blood mononuclear cells (PBMCs) were separated from whole blood by density gradient centrifugation. Using a 50% Percoll solution (density=1.066), PMNs and PBMCs were successfully isolated after centrifugation; the purities of the PMNs and PBMCs were 94.2% and 95.6%, respectively. To evaluate the function of isolated ferret PMNs, we measured the superoxide generation with a MCLA-dependent chemiluminescence assay. The isolated ferret PMNs
more » ... d ferret PMNs responded to phorbol 12-myristate 13-acetate (PMA) with kinetics similar to that of human PMNs. The ferret PMNs did not respond to N-formyl-Met-Leu-Phe (fMLF), unlike human PMNs, which rapidly responded. Thus, authors established a method for the rapid separation of highly purified populations of functional PMNs from the whole blood of ferrets. KEY WORDS: chemiluminescence assay, ferret (Mustela putorius furo), polymorphonuclear cell.
doi:10.1292/jvms.69.1321 pmid:18176035 fatcat:kc5wuzattnd6vhwo2h7p2y66jm