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In this study, a coupled membrane-free microbial fuel cell (MFC) with permeable reactive barrier (PRB) was reported and its treatment performance and electricity generation for acid mine drainage (AMD) were examined. The pilot-scale continuous flow MFC-PRB was operated for five periods at a hydraulic retention time of 48.0 h, and the average sulphate removal percentages of 51.2%, 39.8%, and 33.1% were obtained in effluent of 1,000, 2,000, 3,000 mg/l, respectively. More than 99.5% of the initialdoi:10.15244/pjoes/60891 fatcat:cif5flbatrhapee5owsdue5rra