Cytological and Molecular Characterization of Wheat Lines with Thinopyrum intermedium Chromosome Additions, Substitutions and Translocations Resistant to Barley Yellow Dwarf Virus

H. Sharma, M. Francki, O. Crasta, G. Gyulai, D. Bucholtz, H. Ohm, J. Anderson, K. Perry, F. Patterson
Barley yellow dwarf virus (BYDV) is the most serious viral disease affecting wheat and genes for BYDV resistance have not been found in wheat. BYDV-resistant alien addition and alien snbstitution lines produced from a wheatX Thinopvntm internrediunz (species of Agropvron complex) cross were characterized. Chromosome pairing in the hybrids between two substitution lines showed that they had the same lil. intermedimr chromosome. Likewise. two addition lines involved the same alien chromosome. In
more » ... ien chromosome. In situ hybridization of chromosomes. confirmed that line P29 is a disomic substitution line. Double monosomic seeds and self-pollinated seeds from monosomic addition plants were irradiated to induce translocations between wheat and Th. interrnecliurl chromosomes. Putative translocations were selected on the basis of BYDV resistance and str-rdied by chromosome analysis, Soutlrern hybridization using Thino1svrtun specific probe and RFLP markers. A BYDV-resistant translocation was identified. Barley yellow dwarf virus (BYDV) is a serious disease of wheat (Triticunt aestivum,
doi:10.1508/cytologia.64.93 fatcat:zoak7og5pzdo5jmjr43mk7kcuq