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The gut microbiota plays multiple critical roles in maintaining the health of the host, especially in ruminants; however, our understanding of the process of gut microbiota establishment from birth to adulthood remains limited. Here, we investigated the bacterial ecology in the gastrointestinal segments (rumen, abomasum, duodenum and rectum) of Holstein cows from 1 week to 5 years of age by 16S rRNA gene sequencing to illustrate the dynamic establishment of gastrointestinal microbiota of cows.doi:10.21203/rs.2.9471/v1 fatcat:peehmsw3pfddlkjd6utyi4qmwm